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KMID : 0381219810130010065
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Journal of RIMSK
1981 Volume.13 No. 1 p.65 ~ p.70
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Comparative study on the Methods Estimating the Plasma Level of High-Density Lipoprotein-Cholesterol.
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Abstract
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Correlation between high plasma level of the various lipids and pathogenesis of atherosclerosis, hypertension and ischemic myocardiopathies, due to their precipitation within the vascular wall, has been well documented by various authors. However, it is also well confirmed that there are many other additional risk factors concering pathogenesis of such disease entities. Recently it has been reported that the lowering of plasma level of high-density lipoprotein (HDL)-cholesterol is more closely correlated with development of atherosclerosis and longevity of lifespan than that of plasma level of other lipids.
To estimate the HDL-cholesterol, two steps of the procedure are needed. One of them is purification and the other is estimation. The original and standard method of purification is ultracentrifugation. However, it´s expensiveness and complexity, the routine usage for clinical purpose was postponed uptile more simple and nonexpensive methods were developed. The recently developed methods are precipitation methods using metalic ion and additional agents, such as glycosaminoglycans (heparin or dextrane), EDTA or sodium phosphotungstate.
As estimation methods, there are Lieberman-Burchard method and enzyme assay method. Although the enzyme assay methods using cholinesterolase are well documented as more accurate and precise method, it is more expensive and it has some problems in national economic viepoints in Korea.
To overcome, such economic problems, the author selected a combination using magnesium chloride, sodium phosphotungstate, and Lieberman-Burchard reaction, and compared this test method with enzyme assay methods using 3 different kits and electrophoresis kit,
To compare, the author used the statistical methods, such as simultaneous checking (10 times) in a given day on the pooled serum l , 11 and ]111, daily checking for 7 following days
on the pooled serum IV, V, and VI, and sporadic checking on the 19 cases to estimate the accuracy and precision of the test method and to calculate correlation rates with other methods. As a result, it could be confirmed that the test method had similar accuracy and precision with that of other enzyme assay methods and high correlation rates over the r=0.995 with other methods
Correlation between high plasma level of the various lipids and pathogenesis of atherosclerosis, hypertension and ischemic myocardiopathies, due to their precipitation within the vascular wall, has been well documented by various authors. However, it is also well confirmed that there are many other additional risk factors concering pathogenesis of such disease entities. Recently it has been reported that the lowering of plasma level of high-density lipoprotein (HDL)-cholesterol is more closely correlated with development of atherosclerosis and longevity of lifespan than that of plasma level of other lipids.
To estimate the HDL-cholesterol, two steps of the procedure are needed. One of them is purification and the other is estimation. The original and standard method of purification is ultracentrifugation. However, it´s expensiveness and complexity, the routine usage for clinical purpose was postponed uptile more simple and nonexpensive methods were developed. The recently developed methods are precipitation methods using metalic ion and additional agents, such as glycosaminoglycans (heparin or dextrane), EDTA or sodium phosphotungstate.
As estimation methods, there are Lieberman-Burchard method and enzyme assay method. Although the enzyme assay methods using cholinesterolase are well documented as more accurate and precise method, it is more expensive and it has some problems in national economic viepoints in Korea.
To overcome, such economic problems, the author selected a combination using magnesium chloride, sodium phosphotungstate, and Lieberman-Burchard reaction, and compared this test method with enzyme assay methods using 3 different kits and electrophoresis kit,
To compare, the author used the statistical methods, such as simultaneous checking (10 times) in a given day on the pooled serum l , 11 and ]111, daily checking for 7 following days
on the pooled serum IV, V, and VI, and sporadic checking on the 19 cases to estimate the accuracy and precision of the test method and to calculate correlation rates with other methods. As a result, it could be confirmed that the test method had similar accuracy and precision with that of other enzyme assay methods and high correlation rates over the r=0.995 with other methods
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KEYWORD
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